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Forthcoming Papers…

1. A Comparison of HPLC Analysis of β-Blockers on C18 and C28 Reversed Phases Silica Gel Columns

                                                                                                                

2. New Generation Superficially Porous Silica Gel Columns:Recent Trends in Ultra Fast HPLC 

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The Secret Unrevealed !!! —— The Inside story of Sunshell Superficially Porous HPLC sorbents/columns for Ultra Fast Separation

Imran Ali1 and Vinay D. Gaitonde2

 

1Department of Chemistry, Jamia Millia Islamia, (Central University)

Jamia Nagar, New Delhi – 110025, India

 

2Prochrome India, A/2,Varsha Milan, Sahar Road, Andheri East

Mumbai – 400999, India

 

 

Nowadays, speed and economy are the most important aspects in HPLC analyses as thousands batches are analyzed every month in various industries for quality control. Worldwide, the strong economic pressure of increasing price of many products necessitates the enhancement of analyses throughput in order to reduce manufacturing cost. The production cost can be reduced by reducing HPLC run time and low consumption of costly solvents. Recently, special type of superficially porous silica gel based columns of super fast speed and low solvent consumption have been introduced into the market, which are called new generation columns.

Chromanik Tech. Inc., Japan introduced superficially porous columns under the trade name of Sunshell with good peak shape, high stability and low bleeding. The chemistries of Sunshell columns include C8, C18, RP-aqua, Phenyl. Most recently, Chromanik Tech. Inc., Japan introduce Peptides and Proteins C18 based Wide pore (WP) C18 column with low carbon loading (HWP). The Sunshell sorbents comprises Non Porous Silica core with 1.5 µ of superficially porous RP bonded sorbent layer with narrow well controlled tighter Particle size distribution (dv 90/dv 10= 1.15); offering reinforced column efficiency comparable to sub 2 µ HPLC column performance. This unique configuration offers the performance advantages of sub-2µ particles with backpressures comparable to that of 3 µ Sorbent particles.

The technology embedded in Sunshell sorbent manufacturing consist of single step Co-acervation process resulting in superior homogenous superficially porous Sunshell sorbent unlike similar coating process involving multilayer superficially porous sorbent process. Such Multilayer Porous Coating  being Multi stage process results in Layer to layer diversity and non reproducibility of the particle size throughout the entire coating process. In regards to Sunshell the single step process delivers higher yields and more column to column reproducibility. 

Multi-Layered-Sorbent-Coating-----Single-Layer-Sorbent-Coating

Sunshell columns with Single step Superficial Coating along with unique HMODT Reverse phase bonding are capable of performance at wider pH range and temperature till 80 °C. Sunshell HPLC columns offer good peak shapes, high stability along with low bleeding and these columns can be used for UPLC, LC-MS as well as on conventional HPLC instruments. As of now in core shell category only Sunshell columns have proven stability data at 1% TFA (90 parts) pH 1 at 80 °C and at 10 mM sodium hydroxide (pH 10) at 50 °C.

Taking such predesigned operational matrix of the Sunshell of Columns in considerations, the Chromatographers can look forward for inducting the Sunshell columns in complex chromatographic separations ensuring high quality results.

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Update : Offer Extended till 15th Oct 2012 – “Grab an Exciting Offer for SunShell Columns !!!”

Greetings from Dr. Vinay Gaitonde !!!

 

Considering the adverse HPLC column performance specially with high tailing, Low buffer tolerances, as well as  High temperature column damage resulting in low column life One of the major attribute for these factors is Mobile phase reactivity and High temperature influence on HPLC Sorbent performance affecting the chromatographic performance resulting in shorter column life, increases peak tailing, loss of resolution .It may be noted that very few columns can withstand aggressive M. Phase conditions.

Considering these challenges for chromatographers in terms of High pH and temperature performance in HPLC methodologies, Biotech along with ChromaNik Japan have introduced Sunniest range of HPLC columns and SunShell range of HPLC columns for these demanding conditions. These HPLC chemistries are patented by ChromaNik Technologies in Sweden, USA and Japan .Both Sunniest and SunShell columns are globally promoted by Biotech AB and ChromaNik Japan .The salient feature of the Sunniest and SunShell range of Columns being tailing free chromatography with and long column life along with its ability to ensure consistent performance at elevated pH and temperature conditions.

For greater overview of the product range may we suggest you to visit our web site www.prochrome.net, wherein you will find the relevant information of the Sunniest and SunShell columns along with Biotech product range. The website offers significant inputs in regards to tech support and practical suggestions leading to quality Chromatographic analysis.

The SunShell columns do offer high quality chromatographic performance under demanding aggressive conditions, such as high pH tolerances (pH1-10) and temperature endurance till 80 deg C. The relevant stability data is available on our website www.prochrome.net, The SunShell columns do incorporate the Superficially porous core shell technology with 2.6µm particle size and results in faster analysis, saving of Solvents yet ensuring compliance to stringent Pharmacopeal system suitability requirements These SunShell columns offer UPLC performance using the conventional HPLC instruments and minimal saving of 60-70 % analysis time and solvents. On performance front the SunShell columns offer superior performance in comparison to Agilent Poroshell, Thermo Accucore, Phenomenex Kinetex brand and Supelco Ascentys express Core shell HPLC columns in cost effective manner.

 

In consideration of the superior quality performance reinforced by high quality results and long column life (along with considerable saving of HPLC analysis time,) we are pleased to introduce a special limited period offer for SunShell columns to the users in India.Pl refer below Table No 1 for the various Chemistries and dimensions of SunShell columns. Under the specially discounted scheme ( limited period offer valid till 31st May 2012- code pr1/2012) the user is entitled for 25 % discount on the Japanese list prices and on Order confirmation for any four columns from the table No. 1(Attached), ChromaNik Technologies will offer one ultra low volume Guard column holder(part No. CBGAAC) along with four Replacement RP cartridges(CBGAAH) List Price 41,000/-Jap yen .

Many reputed pharmaceutical companies such as Ranbaxy, Daiichi, Wockhardt, Cipla and Lupin labs have inducted Sunniest and SunShell columns for their analytical work for such demanding applications with reliability and endurance.

This special offer code pr1/2012 is made by ChromaNik technologies and the buyer need to place the P. Order  in the name of ChromaNik Technologies, Osaka, Japan using Jap yen as currency. Needless to say Biotech India and Prochrome India will give relevant tech support to users.
If need be there for any additional information, please inform us, we will do the needful from our end,
With Best Regards and we do look forward for your viewpoints and response.

Click the below image to zoom in...

In regard to your choice for analytical method incorporating these chemistries in Dimensions of 2.1 & 3.0 mm i.d., the same can be included in above offer with due confirmation.

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Wishing Long Lives of Superficially Porous Particles HPLC Columns

1. Superficially Porous Particles HPLC Columns

Nowadays, scientists, academicians, industrialist and government authorities are looking for high speed analyses due to economic pressure all the world over. This requirement has been fulfilled by newly developed superficially porous particles columns (SPPCs). Generally, these particles have 2.7 and 1.7 µm as overall and central solid core diameters with a 0.50 µm outer thick porous layer with 90.0 Ǻ pores size. These porous silica gel particles are prepared by Fused Core Technology. The porous particles were prepared of various types of silica gels such as C8 and C18 and RP Amide and Hilic. The beauty of these columns is that in spite of small particle size they can be used with normal HPLC machine i.e. no need of costly UPLC. Presently, these types of columns are manufactured by Advanced Material Technology, USA (Halo), Supelco, USA (Express), Agilent, USA (Proshell 120), Phenomenex, Madrid Ave, Torrance, CA, USA  (Kinetex) and Chromanik Tech. Inc., Japan (Sunshell).

The pore size in thick porous layer is quite small and there is always risk of blockage of these columns resulting into an increase in back pressure during their uses. These columns are reversed phase and the separation mechanism is as usual of C18 columns i.e. hydrophobic interactions with stationary phase. The choice of mobile phases with these columns is buffers. The most commonly used buffers in HPLC are phospharte, acetate, formate, citrate, borate etc. HPLC separation is improved by using organic modifiers and most commonly used one is acetonitrile. Normally, the constituents of these buffers get precipitated by the addition of acetonitrile in buffers. This precipitation does not affect much in normal C18 columns due to large pore size but blocks superciliously porous silica gel particle based columns due to small pore size. Consequently, the back pressure starts to increase with respect to the operational time. It results in to the blockage of column after about 100 runs; depending on the mobile phase, duration of experiment and other experimental conditions. This results into a need of costly UPLC due to an increase in back pressure, poor reproducibility and finally the risk of column damage. During our experiments, we observed certain phenomenon and reached on some conclusions for reproducible results and long lives of these columns. Some most important precautions to achieve reproducible results and long lives are summarized below.

2. Precautions for Reproducible Results and Long Lives of SPPCs:

The precipitated buffer constituents appear to become trapped in small pore size of SPPCs. The following precautions should be taken when working these SPPCs:

2.1 The mobile phase should be prepared daily; especially for buffers having

acetonitrile.

2.1  The normal washing procedure is not sufficient to dissolve and remove the trapped buffer constituents. Therefore, a little rigorous washing is required to remove precipitated buffer ingredients. After the run of about 10-12 hrs time, the column should be washed with water at 0.5 mL/min. flow rate for 1.0 h followed by further washing at 1.0 mL/min at 1 h time.

2.3 Store column in water-acetonitrile (40:60, v/v) mobile phase.

3        Conclusion:

The reproducibility and life of a column is very important aspect in HPLC analyses. The reproducibility is the scientific requirement while life of column controls the economy of the method. Both are important in research and development and routine analyses in the industries. Therefore, the proposed precaution protocol is very important issue in SPPCs.

 

*Imran Ali and Vinay D. Gaitonde

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MOD 2003 Stand Alone Degasser

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